MST43

The 43rd International Conference of the Microscopy Society of Thailand
June 8 - 11, 2026, The 80th Anniversary of Kasetsart University Hall, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, Thailand



 

MST43 Speaker Detail



Prof.Dr.Wen-Hsin Chung
Invited Speaker
Abstract Title: Application of SEM-based electron probe analysis for detecting hevey metal in Himematsutake
Session: Biological Science
From: Department of Plant Pathology, National Chung Hsin University, Taiwan

Biography

Wen-Hsin Chung, Ph. D. 

Department of Plant Pathology, National Chung Hsing University

145 Xingda Road, Taichung 402, Taiwan, ROC

Cell phone: +886-918595708

Email: wenchung@nchu.edu.tw

Working Experience

2022Director of Agricultural Extension Center and Chair of Master Program for Plant Medicine and Good Agricultural Practice

2016―2019  Director of Agricultural Products Approval and Certification Center

2014―Current  Professor, Department of Plant Pathology, National Chung Hsing University, Taiwan, ROC

2010―2014    Associate Professor, Department of Plant Pathology, National Chung Hsing University, Taiwan, ROC

2005―2010    Assistant Professor, Department of Plant Pathology, National Chung Hsing University, Taiwan, ROC

2004―2005    Postdoctoral Researcher, National Institute for Agro-Environmental Sciences

 

1995―1999    Principal Research Assistant, Asia Vegetables Research Development Center (AVRDC), Taiwan, ROC 

Education

2000―2004    Ph.D., Doctoral Program in Agricultural Sciences, Tsukuba University, Japan

1991―1993    M.S., Department of Plant Pathology, National Chung Hsing University, Taiwan, ROC

 

1987―1991    B.S., Department of Plant Pathology, National Chung Hsing University, Taiwan, ROC

Research Area

 

The research focus on the biological control, such as seed treatment using microorganisms for diseases control and growth inducing. Another is seed-borne diseases, including Fusarium oxysporum, F. solani and Alternaria spp. in cucurbit.

Honors and Awards

                            Academic award of Chinese Sustainable Agriculture Association

 

                            Academic award of Taiwan Plant Pathology Society

Activities in Professional Societies

l   Member of American Phytopathological Society since 2015

l   Co-Chairman of section “Rust fungi” of Asia Mycological Congress 2017

Published Articles (Recent 3 years)

1.  Namisy, A., Chen, S. Y., Sritongkam, B., Unartngam, J., Thanarut, C. and Chung, W. H.* (2025). Evaluation of luffa rootstocks to improve resistance in bitter gourd (Momordica charantia L.) against Fusarium wilt. Plants 2025, 14, 1168 https://doi.org/10.3390/plants14081168.

2.  Lo, P. H., Huang, J. H. Chang, C. C., Namisy, A. Chen, C. Y. and Chung, W. H.* (2025). Diversity and characteristics of Fusarium solani species complex (FSSC) Isolates causing collar rot and fruit rot of passion fruit in Taiwan. Plant Disease 109: 170-182.

3.  Hsu, Y. C., Wang, C. J. and Chung, W. H.* (2025). First report of snow bush wilt caused by Ralstonia pseudosolanacearum (Ralstonia solanacearum phylotype I) in Taiwan. Crop Protection 190: 107070 https://doi.org/10.1016/j.cropro.2024.107070

4. Namisy, A., Chen, S. Y., Huang, J. H., Unartngam, J., Thanarut, C. and Chung, W. H.* (2024). Histopathology and quantification of green fluorescent protein tagged Fusarium oxysporum f. sp. luffae isolate in resistant and susceptible Luffa germplasm. Microbiology Spectrum (doi/epub/10.1128/spectrum.03127-23)

5.  Chen, Y. J., Chen, H. J. and Chung, W. H.* (2023). Endophytic Fungal Diversity in Cirsium kawakamii from Taiwan. Journal of Fungi 9, 1076. https://doi.org/10.3390/jof9111076 (SCI)

6.  Lo, P. H., Lai, Y. T., Ko, Y. T., Kuo, C. C. and Chung, W. H.* (2023). First Report of Target Spot Disease of Strawberry Caused by Corynespora cassiicola in Taiwan. Plant Disease https://doi.org/10.1094/PDIS-09-22-2048-PDN. (SCI)

7.  Wu, C. C., Shen, Y. M., Teng, Y. C. and Chung, W. H.* (2023). First report of lisianthus wilt caused by Fusarium oxysporum f. sp. eustomae in Taiwan. Crop Protection 17, 106298. (SCI)

8.  Chu, S. C., Lin, K. H., Lin, T. C., Thanarut, C. and Chung, W. H.* (2022). Sensitivity of Colletotrichum gloeosporioides species complex (CGSC) isolated from strawberry in Taiwan to benzimidazole and strobilurin. J. Pestic. Sci. 47(4), 172–183. (SCI)

9.  Lin, K. H., Lai, Y. C., Lin, Y. P., Ho, M. W., Chen, Y. C. and Chung, W. H.* (2022). Antifungal susceptibility of the clinical and environmental strains of Cryptococcus gattii sensu lato in Taiwan. Mycoses 66: 13–24. (SCI)

10. Zhou, Z. T., Tsan, W. C., Chung, W. C. and Wang, C. L. (2022). First report of mango leaf blotch caused by Pseudoplagiostoma mangiferae in Taiwan. Plant Disease https://doi.org/10.1094/PDIS-04-21-0778-PDN

11. Sritongkam, B., Sun, P. L., Lo, P. H., Shen, Y. M., Wang, C. J., Unartngam, J. and Chung, W. H.* (2022). Novel causative agents of Fusarium solani species complex causing stem and fruit rot in cucurbit in Taiwan. Journal of Phytopathology 170: 462-478. (SCI)

12. Lin, Y. Y., Lu, S. H., Gao, R., Kuo, C. H., Chung, W. H., Lien, W. C., Wu, C. C., Diao, Y. and Wang, H. M. D. (2021). A Novel Biocompatible Herbal Extract-Loaded Hydrogel for Acne Treatment and Repair. Hindawi ID 5598291, https://doi.org/10.1155/2021/5598291 (SCI)

13.  Lin, K. H., Lin, Y. P., Ho, M. W., Chen, Y. C., Chung, W. H.* (2021). Molecular epidemiology and phylogenetic analyses of environmental and clinical isolates of Cryptococcus gattii sensu lato in Taiwan. Mycoses 64: 324-335. (SCI)

14.  Dlamini, Z. M. and Chung, W. H.* (2021). Evaluation of endophytic Bacillus and Streptomyces against Curvularia lunata and control efficacy on maize. Journal of International Cooperation 16: 121-136.

15.  Yeh, C. C., Wang, C. J., Chen, Y. J., Tsai, S. H., Chung, W. H.* (2021). Potential of a volatile-producing endophytic fungus Nodulisporium sp. PDL-005 for the control of Penicillium digitatum. Biological Control. 152: 104459. (SCI)

16.   Huang, C. J., Chung, W. C., Chung, W. H.* 2021. Evaluation of two essential oils on mycelial inhibition of fungal pathogens and control of gray mold in strawberry in Taiwan. J. Plant. Med. 63(3): 19-30.

 

17.  Lin, W. J., Tzou, Y. M., Wang, C. J., Chung, W. C., Chung, W. H.* 2021. Evaluation of Biochar Materials for Controlling Bacterial Wilt of Tomato in Taiwan. J. Plant. Med. 63(3): 9-18. 

18. Yeh, C. C., Wang, C. J., Chen, Y. J., Tsai, S. H., Chung, W. H.* (2021). Potential of a volatile-producing endophytic fungus Nodulisporium sp. PDL-005 for the control of Penicillium digitatum. Biological Control. 152: 104459. (SCI) 

19. Huang, C. J., Chung, W. C., Chung, W. H.* 2021. Evaluation of two essential oils on mycelial inhibition of fungal pathogens and control of gray mold in strawberry in Taiwan. J. Plant. Med. 63(3): 19-30. 

20. Lin, W. J., Tzou, Y. M., Wang, C. J., Chung, W. C., Chung, W. H.* 2021. Evaluation of Biochar Materials for Controlling Bacterial Wilt of Tomato in Taiwan. J. Plant. Med. 63(3): 9-18.

21.  Wang, C. J., Thanarut, C., Sun, P. L. and Chung, W. H.* 2020. Colonization of human opportunistic Fusarium oxysporum (HOFo) isolates in tomato and cucumber tissues assessed by a specific molecular marker. Plos one 15: e0234517. (SCI)

22.  Lin, K. H., Lin, Y. P., Ho, M. W., Chen, Y. C. and Chung, W. H.* 2020. Molecular epidemiology and phylogenetic analyses of environmental and clinical isolates of Cryptococcus gattii sensu lato in Taiwan. Mycoses 64: 324–335. (SCI)

23.  Chen, Y. J., Lin, Y. S., Pan, H. R. and Chung, W. H.* 2020. Distribution and multiplication of Ralstonia solanacearum strain race 1 biovar 4 in vegetable sweet potato cuttings. Journal of Phytopathology 168: 36-46. (SCI)

 

 

Lecture Summary

Himematsutake (Agaricus blazei Murill) is a popular edible mushroom in the world. However, the accumulation of heavy mental in Himematsutake is risk for food safety.  Several methods can be used to detect the accumulation of heavy metals in biological materials; however, these methods are time consuming. For detecting the heavy metals and its distribution in fruit body of Himematsutake rapidly, the scanning electron microscopy-energy dispersive X-ray spectrometer (SEM-EDX) was used to analyze eight heavy metals of Cr, Ni, Cu, Zn, As, Cd, Hg and Pb in pileus and stipe of fruiting body by pieced and powdery types. Moreover, the atomic percentage of these heavy metals in pileus and stipe were be confirmed by ICP-MS. Four Himematsutake cultivars collected from Taichung, Changhua, Nantou and China. The results showed that the accumulation and distribution of heavy mental associated with cultivars and cultural areas of Himematsutake. Comparing the difference of heavy metals accumulation in basdiospore, pileus and stipe, the heavy metals accumulation in pileus is higer than basidiospore and stipe, especially, Zn, Hg and Pb. In this study, we used ICP-MS to confirme the heavy mentals content. Results indicated that accumulation and distribution of Cu, Zn and Pb in Himematsutake analyzed by SEM-EDX are similar with ICP-MS analysis. In additon, powdry type of Himematsutake anlyzed by SEM-EDX showed better accuracy than peced type.

 

 


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