MST43

Sciatic nerve injury (SNI) is neurological condition that affecting the motor and sensory functions of muscles, characterized by the Wallerian degeneration and regeneration myelin axon. Motor neuron injuries result in muscle weakness and atrophy, sensory neuron damage impairs sensation, and autonomic injuries lead to organ dysfunction. This study investigates the potential effects of Clitoria ternatea nanoemulsion with different dosage (250 mg/kg, 500 mg/kg and 1,000 mg/kg) on Sprague Dawley (SD) rats that induced with sciatic nerve crush injury. Sciatic nerve crush injury was induced on SD rats using modified Watchmaker’s forceps for 15 seconds. On day 14 and 28, behaviour analysis of motor and sensory test was evaluated to assess the functional recovery. On day 28, the muscles were weight and stained with Haematoxylin & Eosin, while sciatic nerve were stained using Cresyl violet, Toluidine blue and TEM. The outcome of the treatment shown an improvement in functional recovery in the motor and sensory test. Additionally, the histomorphometry examination shows the same pattern as the behaviour analysis, increased in the degree maturity of the regenerated nerve fibres which resulting more axonal growth and thicker myelin. In conclusion, CT nanoemulsion treatment in high dose treatment shows promising potential treatment in treating sciatic nerve injury by improving functional recovery and myelin integrity.